摘要 为探明霉变红鱼干中黄曲霉B1(AFB1)和T-2毒素(T-2)污染的潜在危害与肠道微生物的关联性,采用灌胃法对小鼠染毒,采用Illumina-MiSeq高通量测序技术分析微生物群落结构和组成变化,采用血液分析仪测定小鼠红细胞与白细胞数量以及血红蛋白浓度,采用酶标法测定小鼠2种肝功能酶活(谷草转氨酶、谷丙转氨酶),并分析肠道菌群变化与这些潜在危害的关联性。结果表明,AFB1能显著提高拟杆菌门的普雷沃氏菌科、鼠杆菌科、厚壁菌门的梭菌科的相对丰度,降低厚壁菌门的乳杆菌科与变形菌门的产碱杆菌科的相对丰度;而T-2显著增加厚壁菌门的乳杆菌科丰度,显著降低鼠杆菌科、产碱杆菌科丰度,这些菌群的丰度变化均被证实与肝炎、血液与免疫疾病相关联。AFB1毒素组显著降低小鼠白细胞数量(P<0.05),而红细胞数量与血红蛋白变化不显著(P>0.05),说明AFB1具有免疫毒性;T-2引起小鼠3项血液指标全部显著降低(P<0.05),说明T-2具有较强的免疫毒性与血液毒性。AFB1毒素组的小鼠肝脏内谷草转氨酶(AST)/谷丙转氨酶(ALT)的比值大于1,表现明显的肝炎症状;而T-2毒素组小鼠肝脏的AST/ALT比值小于1,肝损伤不明显。红鱼干中AFB1和T-2毒素引起肠道菌群丰度变化与其表现出的肝炎、血液毒性与免疫毒性相关,说明鱼干中AFB1与T-2残留危害还可能与诱变肠道微生物有关。 In order to investigate the potential harmfulness of Aflatoxin B1(AFB1)and T-2 toxin(T-2)contamination in mouldy dried Lutjanus erythopterus and their relationship with intestinal microorganisms,this study used gastric lavage method to infect mice.Illumina-MiSeq high-throughput sequencing technology was used to analyze the changes of microbial community structure and composition,and hematology analyzer was used to determine the erythrocyte,leucocyte and hemoglobin of mice.The activity of two liver enzymes(aspartate aminotransferase and alanine aminotransferase)in mice was determined by enzyme labeling method,and the relationship between the changes of intestinal flora and these potential hazards was analyzed.The results showed that AFB1 significantly increased the relative abundance of Prevotellaceae and Muribaculaceae of Bacteroides,Clostridiales of Firmicutes,and decreased the relative abundance of Lactobacillaceae of Firmicutes and Alcaligenaceae of Proteobacteria,while T-2 significantly increased the abundance of Lactobacillaceae of Firmicutes.However,the abundance of Muribaculaceae and Alcaligenaceae was significantly reduced.The changes of the abundance of these bacteria were confirmed to be associated with hepatitis,blood and immune diseases.AFB1 group significantly reduced the number of leucocyte(P<0.05),but the number of erythrocyte and hemoglobin did not change significantly(P>0.05),indicating that AFB1 had immunotoxicity;T-2 group caused three blood indicators of mice to decrease significantly(P<0.05),indicating that T-2 had strong immunotoxicity and hematotoxicity.The ratio of aspartate aminotransferase(AST)/alanine aminotransferase(ALT)in mice liver of AFB1 group was more than 1,showing obvious hepatitis symptoms,while the ratio of AST/ALT in mice liver of T-2 group was less than 1,and liver injury was not obvious.Abundance changes of intestinal flora caused by AFB1 and T-2 toxin in dried Lutjanus erythopterus were associated with hepatitis,blood toxicity and immunotoxicity,suggesting that the residu
出处 《中国食品学报》 EI CAS CSCD 北大核心 2021年第4期131-140,共10页 Journal of Chinese Institute Of Food Science and Technology
基金 国家自然科学基金面上项目(31871898) 广东省现代农业产业技术体系创新团队建设专项(2019KJ149)。
关键词 红鱼干 AFB1 T-2毒素 特殊毒性 肠道菌群 dried red fish aflatoxin B1(AFB1) T-2 toxin special toxicity intestinal flora
