摘要 磷脂酶D是一类酯键水解酶,能够生物转化卵磷脂生成磷酯酰丝氨酸,在食品及医药领域有重要应用。为高效获得磷脂酶D,实现生物转化生产磷酯酰丝氨酸,以前期获得的1株具有磷脂酶D活性的蜡状芽孢杆菌ZY12为发酵菌株,通过单因素试验,确定影响蜡状芽孢杆菌产生磷脂酶D的条件因素。结果表明,20 g/L蛋黄、2 mmol/L MgSO4·7H2O、培养温度30℃、pH为7、培养时间36 h,磷脂酶D活性最高为82 mU/mL。同时研究发现,除蛋黄外,大豆卵磷脂、花生卵磷脂、葵花卵磷脂、水溶性卵磷脂均可诱导菌株ZY12产生磷脂酶D,葡萄糖、果糖、麦芽糖则会抑制磷脂酶D的产生。 Phospholipase D(PLD)is a kind of ester hydrolase,which catalyzes biotransformation of lecithin to phosphatidylserine and therefore shows great potential in food and pharmaceutical industries.In this study,a phospholipase D was produced by Bacillus cereus ZY12,and 82 mU/mL of activity was obtained when the strain was cultured under 30℃and pH 7 for 36 h in medium containing 20 g/L of yolk and 2 mmol/L of MgSO4·7H2 O.Further investigation showed that the expression of PLD in this strain could be induced by yolk,lecithin from soybean,lecithin from peanut,and lecithin from sunflower,while was completely inhibited by glucose,fructose and maltose.
出处 《食品与发酵工业》 CAS CSCD 北大核心 2021年第9期57-62,共6页 Food and Fermentation Industries
基金 黑龙江省自然科学基金面上项目(C2017066) 2019黑龙江省省属高等学校基本科研业务费科研项目(135409221)。
关键词 蜡状芽孢杆菌 磷脂酶D 磷酯酰丝氨酸 诱导产酶 发酵条件 Bacillus cereus phospholipase D phosphatidylserine induced enzyme fermentation conditions
