摘要 背景与目的:NIMA相关激酶2(NEK2)在多种恶性肿瘤中呈高表达,且与肿瘤进展及不良预后密切相关。然而,NEK2在胰腺癌中的表达及意义尚不清楚。因此,本研究探讨NEK2在胰腺癌组织中的表达及其与患者临床病理因素、预后的关系。方法:收集海南省人民医院肝胆胰外科于2013年1月—2014年12月接受胰腺癌切除术的108例患者组织标本,以及临床病理资料与随访资料。免疫组化法染色检测组织标本中NEK2的表达,分析NEK2的表达与临床病理因素的关系,Kaplan-Meier生存曲线和Log-rank分析及比较不同NEK2表达水平患者无瘤生存率及总生存率差异。Cox风险比例模型分析影响预后的危险因素。结果:在正常胰腺组织中无NEK2表达,108例胰腺癌组织中,NEK2高表达63例(58.3%),低表达45例(41.7%)。NEK2的表达与患者CA19-9水平(P=0.015)﹑TNM分期(P=0.002)﹑肿瘤分化程度(P=0.034)﹑淋巴结转移(P=0.043)明显有关,而与性别﹑年龄﹑吸烟﹑CEA水平﹑肿瘤大小﹑肿瘤部位无明显关系(均P>0.05)。NEK2高表达患者1﹑3、5年无瘤生存率分别为60.5%﹑20.7%、5.2%,NEK2低表达患者1﹑3、5年无瘤生存率分别为75.4%﹑50.6%、24.9%;NEK2高表达患者1﹑3、5年总生存率分别为75.6%﹑42.2%、15.3%,NEK2低表达患者1﹑3、5年总生存率分别为85.7%﹑60.6%、30.1%,NEK2高表达患者的无瘤生存率与总生存率均低于NEK2低表达患者(均P<0.05)。单因素与多因素分析结果显示,TNM分期(P=0.029)﹑淋巴结转移(P=0.016)、NEK2高表达(P=0.032)为无瘤生存率的独立影响因素;TNM分期(P=0.035)﹑分化程度(P=0.042)﹑淋巴结转移(P=0.006)及NEK2高表达(P=0.000)为总生存率的独立影响因素。结论:NEK2高表达是胰腺癌患者预后的独立危险因素,NEK2可作为胰腺癌术后患者预后判断的指标。 Background and Aims:The expression of NIMA-related kinase 2(NEK2)is increased in a variety of malignant tumors,and is closely associated with tumor progression and patients’unfavorable prognosis.However,the expression of NEK2 and its significance in pancreatic cancer are still unclear.Therefore,this study was conducted to investigate the NEK2 expression in pancreatic cancer tissue and its relationship with clinicopathologic factors and prognosis of the patients.Methods:The surgical specimens from 108 patients with pancreatic cancer undergoing pancreatectomy from January 2013 to December 2014 in the Department of Hepatopancreatobiliary Surgery of Hainan Provincial People’s Hospital as well as the clinicopathologic and follow-up data of these patients were collected.The expressions of NEK2 in the tissue samples were detected by immunohistochemical staining.The relations of NEK2 with the clinicopathologic factors were analyzed,and the difference in tumor-free survival and overall survival rates between patients with different NEK2 expression levels were analyzed and compared by Kaplan-Meier survival curve and Log-rank analysis.The prognostic factors were determined by Cox proportional hazard model.Results:There was no NEK2 expression in the normal pancreatic tissue,while in the 108 samples of pancreatic cancer tissue,high and low NEK2 expressions were found in 63 cases(58.3%)and 45 cases(41.7%),respectively.The NEK2 expression was significantly associated with CA19-9 level(P=0.015),TNM stage(P=0.002),tumor differentiation(P=0.034),and lymph node metastasis(P=0.043),but irrelevant to sex,age,smoking,CEA level,tumor size and tumor location(all P>0.05).The 1-,3-and 5-year disease-free survival rates were 60.5%,20.7%and 5.2%in patients with high NEK2 expression,and 75.4%,50.6%and 24.9%in patients with low NEK2 expression,and the 1-,3-and 5-year overall survival rates were 75.6%,42.2%and 15.3%in patients with NEK2 expression,and 85.7%,60.6%and 30.1%in patients with low NEK2 expression,respectively.Both disease-free s
出处 《中国普通外科杂志》 CAS CSCD 北大核心 2021年第3期269-275,共7页 Chinese Journal of General Surgery
基金 海南省自然科学基金资助项目(819QN356,820MS130)。
分类号 R735.9 [医药卫生—肿瘤]
